Tridgett, Matthew Lloyd, James R Kennefick, Jack Moore-Kelly, Charles Dafforn, Timothy R Mutation of M13 Bacteriophage Major Coat Protein for Increased Conjugation to Exogenous Compounds. Experimental methods are presented for the design, construction, and screening of phage-displayed libraries for improved protein display.
By introducing mutations into the anchoring M13 coat protein, protein display levels can be increased by up to two orders of magnitude. This chapter describes a method for increasing levels of protein fusions displayed on the surfaces of M13 bacteriophage particles.
Sidhu, Sachdev S Feld, Birte K Weiss, Gregory A M13 bacteriophage coat proteins engineered for improved phage display.
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